	   SnapGene      ÕgttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttggtaccggccaccATGGGACTCCCAAGGCGGCTGCTGCTGCTGCTGTTGCTGGCGACTACCTGTGTCCCAGCCTCCCAGGGCCTGCAGTGCATGCAGTGTGAGAGTAACCAGAGCTGCCTGGTAGAGGAGTGTGCTCTGGGCCAGGACCTCTGCAGGACTACCGTGCTTCGGGAATGGCAAGATGATAGAGAGCTGGAGGTGGTGACAAGAGGCTGTGCCCACAGCGAAAAGACCAACAGGACCATGAGTTACCGCATGGGCTCCATGATCATCAGCCTGACAGAGACCGTGTGCGCCACAAACCTCTGCAACAGGCCCAGACCCGGAGCCCGAGGCCGTGCTTTCCCCCAGGGCCGTTACCTCGAGTGTGCGTCCTGCACCTCTTTGGACCAGAGCTGTGAGAGGGGCCGGGAGCAAAGCCTGCAATGCCGCTATCCTACAGAGCACTGTATTGAAGTGGTGACCCTCCAGAGCACAGAAAGGAGCTTGAAGGATGAGGACTACACCCGAGGCTGTGGCAGTCTTCCCGGATGCCCAGGCACAGCAGGTTTCCATAGCAACCAGACCTTTCACTTCCTGAAGTGTTGCAACTACACCCACTGCAATGGTGGCCCAGTTCTGGATCTTCAGAGCTTTCCACCGAATGGCTTCCAGTGTTACAGCTGTGAGGGGAACAATACCCTTGGGTGTTCCTCCGAAGAGGCGTCTCTCATCAACTGCCGGGGACCAATGAATCAGTGCCTGGTGGCTACAGGCTTAGATGTGCTGGGAAACCGGAGTTATACCGTAAGAGGCTGCGCCACGGCTTCCTGGTGCCAAGGCTCCCACGTGGCAGACTCCTTCCCGACCCACCTCAACGTCTCTGTCTCCTGCTGCCACGGCAGCGGCTGTAACAGCCCCACAGGGGGCGCCCCCAGGCCAGGCCCTGCTCAACTCAGCCTCATTGCCTCTCTGCTCCTGACCCTCGGACTATGGGGCGTCCTCCTCTGGACCgactacaaggacgacgatgacaagTGAgggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggctGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGtttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacgcggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata  %«         
                        
   	                                                                                    
                                                                                 
               
                                          !                  	                     	            	                  
               
               
                                                                              
                  	   
                                 
      	                                                         
      
                              
         	                  
   
                                                                                                                                     
                           Ï W(1fb>?@EJYZ{Ug WîXz–Øh£>.2g”[d|c	>­å™
 t Ð mAAF¿-h • >Äâ–ó)c‘K†™¥®«	nVí ! ê q{Ù>WÙ/vœ\š ?3•íe	d	™
*j®GÎæö DY€¥_}I	"
ù
?Zf@” Xï—iz¤$/ƒ†h[q~Pþ n"ÕZ]_óB|Àñ.BGƒí“	<
R
Ì
óÏÀ¯ |ÃÈ”†	Ü	ö	
1
x
¥
Æ

 u *K·Ï
P�.i	 $ Ñ Ü™	F¢] z ?ÅÌ®ÇRdš¯ð	l	9
+÷Wî M ŒÓý0‡D™®ÓLs
´
Áë•Ò R ±g—“†­¸È
‡�¦½¬	`
H – 	ÊšãÎâô*ö).’U
oz " ÚXeà&„‘/P«�]ë/
¿ ÿ?øP¯$L8èv› + ë r|úŠ �)ë	E
 
˜¶.Òþ 6 ê RÚ0Nkw
Î+
sÃ< f _Z}×Mîf	e	M
j
‰
{kz¯pÏ  vr–óš
ñ
/HZ÷W} i @pêçÿL -4¸	m
+0 Ž %I�¦Œ…~¾‚É › EûL	J	 	0A ­ Z`#
æ
@NO 6nŸ¤¯
ú
[gÐ%7egxœ•�¥ /;d·Àq»A‚ËÞ ñ‹‘ [ § b`«ˆQiúû	§ ´ YðP˜{¥Ê0‡×\v®ÿ 
ðË W !æUj%{„±ÔbÈiâTrç9	Ê	:ú oBÖ[]—ô}ÑCH„îj	P
S
Í
(}ñ #ù+ö^"`-�½ÉÐÁ   ™ÏÒôµÚ"C/”	°	ûô�ï 0 ë7k·æv�Á²Üò“�(	²	=
&D;�‚° - É+ƒ	–	6
¦
Ç
ý¼ Ã K}Ä¨ü§Ý	÷	
š¸sº… 	 >t›U‡	2
r
y
�ñP
 ‘ �©à4Ñ•Ô»  ¤ ÁÜ¼Ê¢
é§ÙÜAiw sü   v ¹ ô Œø$Ÿ�
9«Ç0Qƒ�/ } +L~‚&P„¸—Í´	Ð
Ø
~Î¹ Ù í BüE4	�	�	Á	G
Þ¿N~j % ¥š	øÿŽ Ò 2gºt21çGöl®à cÝ�Uïõ%æ	£Õœ5 Ôg Q	í	€^ (IÆ¯�:
Ê
Nõô> 4 Œ Ètñ-
Î6,øXï { ¢ ò @Í‚Se›²	h	· 2 ‚ ¹± °*	m	Ñ	
ì
uÒ#o �þ1çèñ,š´ÔMp

(
à
ã
î
w~–- 8 N ¾EÈ2;ÁßîUµ
Ü
+X…ÔFÅÓÝ b Ôí’ tÐìž¤#Ï ˆPm¯´	Â™’q² ( „ M²µ¸»¾ÄTh”*‘®ü¡x�€	¨	­	Î	†
Ã
(p C C€?
a
²å  S o ‡ �9èy‡¹B	o	´Á  ì"˜É4Nˆ§¾á 	,	z	¡	�
©
#AIûU=Ä ãõ+@c?	gpª{ÚÌ  — P¤D› »³Ãÿ*¿Ãô•

Œ
cÃß³%„ Ï:tÚä§7x>F©´ÞU	Ó	V
ì‘ à 
(„­ËÛ³mÏÜ©÷"/“ÑŸÃÆ	 	 s Váò'0�X˜=mäì,f #.‚u…}O^ý!
ø
YeÍ
 # ÛY¶ð-ì…	˜	0
 L Q y Ë­fÍá’­Q…¬Ç(-r¼	8
Àê*Ñ éþùOQd®%Mj	Oªê*
D
Ÿ
µ h ^qi
l
ð
yoé/V š  5H‹	å
MfÿN½wœ
 @az°ÓÇÖhú	9 / ˜ø*êó\Ù!uŒO
'¼%|€î:�  , Â Js�¨ß 3û*q
�™·»rÓº ¸ Ø ì }‹ûž»Ž	F
�
¡
}ªè¾ÆÍ/vÿ} bœæì	›ß H�sg	,
É
ÍtM¶= 7 æ1Ol³ÀToÏ
'
ß
â
W˜,£Ä" B ë!�Sx)	y	§	¨
±T  ß sÙ¦Û6=¨ÂÅ	
‹
bf K P ¬t~Æ|ïW÷
<Xl)dÐãë g `GcyN©g	ù	k
ž
 ¼ —§a[Ø‹åN
|Ž{ºquÞ9€  A åŽ5	f	¦	Š
Þ
°L! x4s	�
ö
W�K8â! : � w×Ø›
œ
ò
ó
ô
õ
0UVuvHIJ7I[ÇÈÕßàáXY~ ; ÆŽ¡ÀÙpwƒÖøZb ˆÀ—›ô·
»
1œ]J\É^ 8j½Ç;w¢ÁA=ðmx±-×ùM ¿ 5S8
lZÊëè [c < ^ É q£¢	 j Å �qÚ„ˆ¸¿ M“ì,Ö"K H ªÊ5
n
¸
Ê_ û €­‰á_8ß	Z
2�‡1]˜ .þÁÃ˜œõ¹	ñ	¼
^Ú‡ è kÈì <xÂ¡B”ñ`�	�“­ d 9¾äJˆ�‚n†Jy–.5:	 � @£�4
?ƒúN ¢Ù&ï>§v²±¿óØÊ À Ý HTFü\M¾mºw	æËì*d ù 6ã	[ é\	 œ ä æ  á’”F ž 9¢		K	
1B _ ª ± ,a´n¸WC"a¤âD2 ® ¼ Ê Õ Ö$
ç
Or  = v[t
AÙP%HRu 49£ã
 7Ûã	
îf– Æ ”�8�õ
 £\r¥ÒR…¹T&†A)  k Æ‰	{
û
\‰ÈÑh�¦žÀ GoFØ	°
hâ�—8¶y¦ “ Í  zÁU”Ó¶^
’
 €ƒÌ¶	þ	-# 0<e¸	rÏí7×LŒ   N«¼BKß~	ê
!?ùò q€¬ËKo±'ƒRû
B
o
¨èË F I \ «a¾6z‰jÕ¹
`½
 ¨ ÷ ’â63(Ds c‹åü	d
 �Rë`¯ý

›«Ì™ µ Ë1ˆâ]w 	à	[
f
 ü Zˆñù™ŠÐ
žˆ2­ Q®|¦Ø9÷3ñ‰Ö^Œ	´ X /ek—4�²™¤sF	Ë	À
3Eû m Šžÿ|…Äj	½
®_Û³ çVÎ&ÂûÉçM	“
bÄ‡ "iÕc�ç;ãU�èö:	º	ò	;ˆ” lí=yÃ×¢•@“a=DIcz…¤ª	¾   é pC=V pì	)F !“\^ò{¿
Q
w
¤
Å
Î
E¡~òŽÎ® ‹ÉÒü/†ÆC˜õ~ÒŽïk	
T

y” * ÷‰#ƒ.Ž(ê	K—¾ÑçuÂ; e ,Y|ÖoL
ˆ
zþK ¬ $úKž£®
/6›È V ¦ ³ .:c¶¿åOpºT_ªaS‡æÊ¦ïÊŠ   Ñõj¶!–,Ž±’¯	±	CÈ � šÐT‚	•	5
üð„O  A�OÀÛ#DÉÌ3	×
¦Û@‚rû ¤Óôÿ$0P	Ôõk­4 1 � '¸°€)	Ð	~
Ñô a çìð+Ç:Þí³	Û
*„E�‘±   n † ´·º½Ã8ç�û3Mw>
…
³'äÀ<Ã ƒ£Úl?¨bwÂþE³¾ÐÝó”ž>	ë©Þ²ÙƒË
 r x UÊ,»„	—	7
e ý]NŠh
eþ´U . · Á × I)ºç½Ìí Þ G€¿nx	&
§
È
S+  @ ä�¨ø	;¹t»ê ~Å‡š	ot‚Œ›\6]	 ¾ Ä 7	·¢É¡
 ú L©ýÞ	
Y
† ç ?¡Øë_Œ3
•’ò¬ � å àâ¡	‘“ ° » u³V8s
ØŸ1GQ 
 œô	Eˆ	×	â	
z
QÇSŒ…( ’ Ÿ Ì ÿ yªÒ]
é
 E ö ‘á5JŠä&‚yA
c
ç2C¼ øªÕ¬ –Í3Žú:£
	E	L	’
­2l  Šû.…’?b£v
£
D xE U ¥ « ² -bµNo¹SX{åé	K
­
týJ
  €Ë#Ö
¥ÚBj3 ` € ÂÝD½ÏÚ
ê¨ÐÝã	 q w Ö (‰%
RT  ? ½ †Œ™:ns�¶¡È5 ¯ º ×ê Ö	
PR‹„‘'0 Ë õ þ x�ù%D	‘
è
¬1©k«  -M„RÎu
Õ
Ù

CÏI > w'…˜m€QŠ4ª&S ~ ,ƒQÑ
Ò
Ó
Ô
BlÃ© \ó\›ê¹Ìµ	X	q	ÚÄ¯ºv ‰ ;`	�	ž	–
ÛÀZR Ú ÍÂ	_ß ° ý·5:Y	r	H
¤ÅäO» î C]ô]œë ºÒF»Ýá¶	5	kw <Ya	‘	—
[S— þ¬Ü€� ¦$&	›	ä	Üùïý gŽ & Š 8Ÿ	
Á ö3¤s;¦èŒ	Ã	%ºËUm¡*¸ Ç 3h•»9u‘]ê6ãþ
S†'Øb Ó Û ž¡ZA2u	Hà‹¯á?B8
 SÎÓ|	5`C÷‡±  l )ÅþòKnÇ&;|
ƒ
¤ÒÆå¼ÁÆ
 Þ~¸6ŠŠÖ� žç	I
«
]§Piž§ dîVXðöI.	Z	s	Š	ü
É¥`6Ÿ¶ ï °wJÀ6	R	Ù	±
Žb·l ^^f�HYpšg»·£	î	ãK”˜z õ	GÓG¼ÚøPhkâ0	i�W_  Dð¨ÕÝì¡ËÞÖù©	"	\	ó9x¡§ ” Î V•g=-e­b	¬T˜ yØ¤›ZH	˜
iõ?“ {Â)JÇÔ‚’	Û	õ	;
Ë
O\
 ™°·	_
³
Gö 5 ßÿ�Í.
7r-ý uLò·	.Y|	 � m„Ï$d�¤ Z  Éáu�­Ýù8	É	ÿ	ùð� AÎ6´åÐÛœ'	i	úð� =
§¦¹ | £ ó %ƒ÷–³	œ	À	8ÝØhM¸ 1f¹s1Tîfå	÷þ�
 3 ‹ ¡ ñ ±ë
5"ón
 ½‘í
vÓ}ÜpÎ ' ƒ LB à+	A	n		 	Í	€
Â
"@úo 'O¬Î²9Cº²!ÂT	Ò	
�Â$ à"-�Ìñ¬µ„',Nq—ü	 
éÌ+ ™ è4­ÒLipÆÕé)
C
ä
ï
Mxn.  ŽÞÿ2÷éò›ú t�	p
Ž
&©»$šÅ|¹ rØr¥²(<§ÁSÄ	

á
ÌVae—¢µ J O –¦`«FbÅ{ŠVä¥	Ý
ÿ
(Ýc 9 ŒŸ¿¿3Ö¶
º
TGÆÔÞa ] È 4Ri¼:v<ï7lk‡°,Y¾ G ’¬~ÉÂà^ë7ð	†Õ!Ù— c ã î‡“œuI4>Œ°9 Ü ã ø GŸ[½¹v	åŸ)EÐ © Ô +`Õm·B!3áâC$t “7F¢[ÅÑá–íµ@¥ TJµÎ}	µ	ý	6>ø p½n°Ô'r ‡QÏêöü


e
ˆš‹³ h‰d�Œææ¿
aÃD“²† Ñ<Å
Uÿz’¾oë<y	©	

Ä
Í½ ) ©ÕR¢'É‡
¥îæš‰Ç N¿Ði S•+ó°Èþ‘2	O	�	®	Ó¬Çq   m … ‚³¶¹¼æ*Æ9ìa¯úý2L=	Ï	}
„
)ƒœÂ ¶ ¹g
d³ X
‹ ²ßóD^7‹	á	Æ×ž« D ÷Ì2I‰ã�x@
\
b
æ  T ‰ú‘z>ä¢è	J
¬
Ÿ p ý é‹ˆC	Õ	
µ¨j [òšéËW	p		k‰Â3¨® ˆ :ŸÑºÜ¶à_	•
^YQŸ #ýX«%	ü� RYé5:@ât	{	‹	ý
$4ÊBŠ×>a7· í}(ÄWñHJm‰-	‚
ª
¦_
µÅ   e¯ïXv§Ü™fIÊÙ÷OjÕø¨¿!	/	[	¢	òJ“Va  fx˜×,£dš¬G	Ú	ô	²
Fh« Y lÞ�Kàtø7	È	qcü{£ 05³äö¥•Ú¿	ö¸ ð &Í±�AŸÁ@	S	Ì	Á
4�|!nÛmÍ  ˜ Ýq×ß !Ñðh«Åù&+À–	�
#L`d™Ä{´ Q¥_‹žª}kÄ¤	ï	¯ Ü– â *F_l¶EZŸ†›¼Ä¸ä•à=´ †gIœo¼é´Íå õû	

¾
=&Š…  Ð;Ïå	¨
8Ôy½ù1QêÈ	1	<	N	í‚ˆ
 ±öòHW
cÅª èÊ=Ûµßã¡V	^	Ô	”
jX WuÛ'ÃüeHè?GWilªÔ$	’` %ÌÖÝŽ¢ß”žÙ«÷¾	Ç	ó	õ
z¢ á )E…Ükµ©ÖÞnÐïªèøÌä%ú"<<‰•  °ñd<0VÓé Ç	#	;	]	© #$®¯j´íî#$Ò×ØªÄÅÆ		»	¼	½	ô!:;y¨  '°  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                            	                                                                                                                                                                                                                                      	                                                                    
                                                                                                        
               
                               	                        
   
                      	                                                   
         
                        I   3                     5                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
            	                                                                                          	                          
                                      
     
           	        
 
        	                 
   
        I 3       5   S2  Sà  Sþ  T   Vâ  Yâ  ]RÞá q	5Ðø%@	W
ð àt,
ß+tHùÊ
„Ëg‡ûûè2	{ 
„¤Ë(@D¯²µ»äè27îüÿx§ã
gé:ß!+nð:? )ððnêíŒ½+v!é!ß!ð+•þ«
¡„—ü�o _á#-�;3b[œÞï¡#/d·q‹ŽwÑ´”Ó‡”ÀñÁ<ÖK™y; Õ‡
ŸÎ/d·qARd‹‹  µ ê Nk²	;�°²	�°ÝýŽ«‘¼  k‘�²	�°ê –Ú‘†¥%	�_f�ítI	@ž£Üø"æ*I��	@
æl	 œ‡h”—Ù(gÀÈ‘ñ¦½ÁHi<UfJè	âý
ad¯8ÎŸªÞßÞïð�ª=d´Ä %Wé–¡ª«	w™F	Ù	;î+Bá—EjÖ‘x¨	É	dùTî?ŸŠ ê k–ÚNk‘†�¥²	;�°H8Ÿª=Ä¡
M4 
ËÄäí7¿pt›Ü
cŸ´ ít ù
ZffwœŒ èñ~êš«…˜m€4°ÀR	Žê <UÕ‡
fJŸ²
á
³¶¹¼ú„
Ÿ¤ ŽŽ¤ }Ï+²	š�Ž°Š w<gÓþÞPna	ç	I
«
þ
HK8—¡]   6	î	 (ŸÌ	Á
npýæ*(Ž‹ýâè	ý
aW jrúæ“[l@¾�ß 3Óºý~Žù
Zf”n¯
g7x¥Ð;ß	!ð«y½&+ê–		cUïõ5?¯8„ ²µ¸»®üÎ	

à
(–• 	Êiâ$ƒÎó)öh.q‘’n]fÖ,¢d«¬ó	«
"]_ÏÀ`C±i$ƒhq]

à
–„ ²µ¸»®üÎ	(EÄ•´¶^
ãõ„ ¯²µ¸»@®ü?	Î	

à
({–õÚÌÐ;fß	!Öð,¢«dy½&+ê–«¬		ó	«
Ðì¤%¦~¾É

Ì Ýù�
#™Ä{€¥}Fá–µ:‹	$Ê·) 'æWî–h£gÔ zÁÓ/4²R¤¯ýo
¨«) p ˆ:'‹	$Êæ·÷éò t&»$Qêü


š»AÞ ñSe%Ìži P{¥p×ë	ð�Ý÷þð��3JY&è6	—
¤bmåÆÛµß^	”
X}îkÏô K¨ü^Œø`$Ý	
�
«ö0É…f _Mfj
ž]Pžë	P{¥×ð^`öÉi p¯@?	{õÚÌ÷éòQê tü


&»$šd nyÚ ß’·3 ‹ ¡ ñ ±ë
5"ónð &Í±�AŸÁ@	S	Ì	Á
4�|!nÛmÍ(IÆ¯�:
Ê
Nõô>‘ �©à4Ñ•Ô»B ë!�Sx)	y	§	¨
±T2 ‚ ¹± °*	m	Ñ	
ì
uÒ#o¶ ¹g
d³6nŸ¤¯
ú
[gÐ%7egxœ•�¥– 	ÊšãÎâô*ö).’U
ozfx˜×,£dš¬G	Ú	ô	²
Fh«c ã î‡“œuI4>Œ°9 B R – ß 	Êë!s�š±ÙãSg—¦ÎÛâô*6x“ö)†­¸È
).=’¨Â‡�¦½	y	§	¬	Å	
U
`
‹
¨
Hbfo±Tzc � Ü ã å ç ø ?GŸ¡Øãëàâ[ î‡“œ½¡_uŒ¹	v	3
I‘“•å4>Œ’Ÿ°ò)9E¬Ð0<e¸	rÏí7×LŒa çìð+Ç:Þí³	Û
*„E�‘±< ^ É q£¢WuÛ'ÃüeHè?GWilªÔ$	’`" ÚXeà&„‘/P«�]ë/
¿ ? ½ †Œ™:ns�¶¡È5# B 0<e¸ç	rÛë!V�Î&SYÂûx¶É)Ïçíð-ì	M	y	…	˜	§	0
“
¨
7bÄ±×TL‡Œþ¬Ü€�   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ê<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="Plaur" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1937" maxFusedRunOn="1937"><Segment range="930-1910" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MGLPRRLLLLLLLATTCVPASQGLQCMQCESNQSCLVEECALGQDLCRTTVLREWQDDRELEVVTRGCAHSEKTNRTMSYRMGSMIISLTETVCATNLCNRPRPGARGRAFPQGRYLECASCTSLDQSCERGREQSLQCRYPTEHCIEVVTLQSTERSLKDEDYTRGCGSLPGCPGTAGFHSNQTFHFLKCCNYTHCNGGPVLDLQSFPPNGFQCYSCEGNNTLGCSSEEASLINCRGPMNQCLVATGLDVLGNRSYTVRGCATASWCQGSHVADSFPTHLNVSVSCCHGSGCNSPTGGAPRPGPAQLSLIASLLLTLGLWGVLLWT"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1937" maxFusedRunOn="1937" isFavorite="1" detectionMode="exactProteinMatch"><Segment range="1911-1934" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="2" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="924-929" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="774-792" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="753-771" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="attB" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2196-2229" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;phage Ï†C31 integrase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal attB site for the Ï†C31 integrase (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10801973&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10801973&quot;&gt;Groth et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3850-3954" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="548-751" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1969-2193" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="168-547" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2230-2818" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3780" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2989-3780" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="3781-3849" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  È<Notes>
<UUID>7c749386-4fb7-4f2b-9f2f-21300c018cf6</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>mi-pcDNA5-MPLAUR-FLAG</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="6:51:44">2022.12.15</Created>
<LastModified UTC="22:16:46">2025.9.20</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO ÿþ               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
